As of Thanksgiving, Instacart users across 35 states in 5,000 new zip codes will be able to order Aldi products online.
Through the national rollout, the partnership will now cover 75 major markets, including San Diego, New York City, Miami, Raleigh and Minneapolis.
"ALDI is a pioneer and a leader in creating a shopping experience that works with people's busy lives," said Jason Hart, CEO of ALDI U.S.
"Our partnership with Instacart and the expansion of our e-commerce options are more ways we are meeting the growing needs of today's shopper, who wants high-quality food at unbeatable prices."
The German grocery firm piloted the Instacart partnership last year in Atlanta, Chicago, Dallas and Los Angeles.
In August, the grocer also announced that it will spend around $4bn to remodel and expand its US chain to 2,500 store by the end of 2022.
Additionally, Aldi said it would roll out scores of new products, with plans to increase its product assortment by 20%.
"The response to the ALDI pilot programs in Atlanta, Dallas, Los Angeles and Chicago have been overwhelmingly positive. Bringing delivery to ALDI stores across the U.S. enables Instacart to grow its base of customers and introduce even more people to same-day grocery delivery," said Apoorva Mehta, Founder and CEO of Instacart.
"Like ALDI, Instacart is focused on delivering value and making quality food accessible and affordable to everyone. We're excited to work together to further innovate the shopping experience with fast delivery and low prices for ALDI customers."